Stir in other ingredients until fully dissolved.Using a jar funnel and a measured ladle or pitcher, fill jars not more than halfway.Drop a stir bar or other agitator into each jar.Wipe up any drips from the rim of the jars. Put on airport lids and screw down rings until snug. Cover jar lids with foil to keep filters dry, and load into PC. Include any syringes or other tools in need of sterilization, wrapped in foil.Sterilize for 15-20 minutes at 15 psi. Longer cooking will caramelize and toxify the sugars and melt the syringes.
This biopsy technique works well with most fleshy mushrooms, though tough or tiny mushrooms can be nearly impossible to catch This way. Waterlogged mushrooms should be allowed to dry out a little in the fridge first. Dry mushrooms can be soaked in 3% hydrogen peroxide to rehydrate. For improved chances, repeat process with multiple jars of media.
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You’ll need: a mushroom, a sterile syringe fitted with a 16-gauge needle, a sterilized jar of water with an airport lid, a jar of sterilized LC medium with an airport lid, alcohol in spray bottle, alcohol prep pads, cotton balls, tweezers or knife (optional), permanent marker.
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Once a spore syringe is transferred to LC, grain, or substrate, the result will be multispore germination, which leads to multiple genets growing together. Choice fruits from this lineage can be tissue-cultured to isolate a good strain.
You’ll need: a spore print in a bag (see “Spore Prints,” above), a sterilized water jar with airport lid, cotton balls, a sterile syringe, alcohol spray, and an alcohol flame or substitute.
You’ll need: spore/LC syringe and alcohol flame or extra sterile needle, OR sterile syringe and LC jar; sterile LC media jar; alcohol spray; and cotton balls. If using pre-made spore or LC syringe, skip steps 5 and 6 and do step 7.
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Gather all tools and supplies on a clean surface.
You’ll need: an aseptic transfer space (i.e., still air box), a sterile syringe and needle, a sterilized jar of water fitted with an airport lid, a mycelial culture on agar (in either a Petri dish or a culture tube [slant]), a jar of sterilized LC medium, alcohol spray, cotton balls, plate wrapping material, and a permanent marker.
Excerpted from DIY Mushroom Cultivation, by Willoughby Arvalo. Published by New Society Publishers © 2019 by Willoughby Arvalo. All rights reserved.
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Categories: Recipe
This post was last modified on 06/11/2023 01:48
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